Linsmaier and Skoog (LS) Media is a plant growth medium formulated to improve the nutritional requirements specifically for tobacco cultures. Developed by Linsmaier and Skoog in 1965, it’s a widely used alternative to Murashige and Skoog (MS) media, particularly for micropropagation, organ culture, callus culture, and suspension culture.
Here’s a table outlining the basic LS media formula (which is very similar to MS media):
| Component | Concentration (mg/L) |
|---|---|
| Macronutrients (same as MS media) | |
| Potassium Nitrate (KNO3) | 1900 |
| Ammonium Sulfate ((NH4)2SO4) | 700 |
| Calcium Chloride (CaCl2.2H2O) | 440 |
| Magnesium Sulfate (MgSO4.7H2O) | 370 |
| Potassium Phosphate Dibasic (K2HPO4) | 170 |
| Micronutrients (mostly same as MS media) | |
| Manganese Sulfate (MnSO4.H2O) | 20 |
| Zinc Sulfate (ZnSO4.7H2O) | 8 |
| Boric Acid (H3BO3) | 6.2 |
| Sodium Molybdate (Na2MoO4.2H2O) | 0.25 |
| Cupric Sulfate (CuSO4.5H2O) | 0.05 |
| Cobalt Chloride (CoCl2.6H2O) | 0.05 |
| Ferrous Sulfate (FeSO4.7H2O) | 27.8 |
| Vitamins (modified from MS media) | |
| Myo-Inositol | 100 |
| Thiamine Hydrochloride (B1) | 10 |
| Pyridoxine Hydrochloride (B6) | 1 |
| Nicotinic Acid | 0.5 |
| Glycine | 2 |
| Carbon Source (same as MS media) | |
| Sucrose | 30,000 (Note: This concentration can vary depending on the application) |
| Other (same as MS media) | |
| Agar (g/L) | 8 (optional) |
Note: The main difference between LS and MS media lies in the vitamin concentration. LS media has a higher concentration of Thiamine compared to MS media.