Exploring Quoirin and Lepoivre (QL) Medium: A Key Tool in Plant Tissue Culture The ability to culture plant tissues in controlled environments has transformed the fields of botany, agriculture, and biotechnology. Alongside advancements in micropropagation and genetic engineering, tissue culture techniques now play an essential role in plant breeding, conservation, and scaling up the propagation … Read more
Unlocking Plant Growth Potential: Understanding Knudson C Medium When it comes to plant propagation and in vitro growth, tissue culture media forms the foundation for successful cultivation. Amongst the array of specialized culture media, Knudson C Medium holds a prominent spot, especially for the growth of delicate and nutrient-sensitive plants like orchids. Understanding what Knudson … Read more
Exploring Nitsch and Nitsch (NN) Medium: A Cornerstone in Plant Tissue Culture Plant tissue culture has revolutionized the field of botany and agriculture, enabling researchers to propagate plants under controlled conditions. One of the many pivotal tools in this field is the culture media, which acts as a nutrient-rich environment for plant cells, tissues, or … Read more
White’s Medium: A Key Medium in Plant Tissue Culture Tissue culture is a widely used technique in plant biology and biotechnology, allowing the growth and propagation of plants in controlled, sterile environments. One of the cornerstone solutions that support plant growth in vitro is the plant tissue culture medium. Today, we’ll explore one classic tissue … Read more
Title: Schenk and Hildebrandt (SH) Medium: A Comprehensive Guide in Plant Tissue Culture In the field of plant biotechnology, the development of refined tissue culture media has proven indispensable for the success of in vitro plant growth, propagation, and research. One such seminal medium is the Schenk and Hildebrandt (SH) Medium, which has been instrumental … Read more
Murashige and Skoog (MS) Medium: The Gold Standard for Plant Tissue Culture Introduction Murashige and Skoog (MS) Medium, developed by plant physiologists Toshio Murashige and Folke K. Skoog in 1962, has become an essential cornerstone in the field of plant tissue culture. Widely regarded as the “gold standard,” MS medium supports in vitro plant growth … Read more
Linsmaier and Skoog (LS) Medium: A Foundation for Plant Tissue Culture In the world of plant biotechnology and tissue culture, the culture medium serves as the lifeblood for in vitro plant growth. One of the most influential and frequently cited plant culture media is Linsmaier and Skoog (LS) Medium. Developed in 1965 by Erich Linsmaier … Read more
Understanding Gamborg’s B5 Medium and Its Applications in Plant Tissue Culture Plant tissue culture has become an indispensable tool in modern plant biotechnology, aiding the controlled growth of plant cells, tissues, or organs in an artificial environment. One of the critical components of successful in vitro plant cultivation is the culture medium, which provides the … Read more
The bromeliad Tillandsia eizii is a striking species with large, colorful, and persistent inflorescences that can reach 1 m in length. The value of this plant as an ornamental and its importance in cultural and religious activities has led to its over-collection in the wild. Clonal propagation via tissue culture may be a means to repopulate native stands while meeting the demands for this species as an ornamental and ceremonial plant. Adventitious bud proliferation was induced from axenically germinated seedling material. Parameters evaluated were the age of explant material at the time of transfer onto bud-induction medium, the concentration of plant growth regulators, and the period of exposure to induction medium. Light and scanning electron microscopy (SEM) established the origin and development of buds. Twelve-week-old seedling explants rapidly initiated adventitious buds after a 30-d induction period on shoot-initiation medium. Adventitious buds were induced in 40% of the explants placed on media with 2 mg l21 6-benzylaminopurine (BA) (8.88 mM) plus 0.1 mg l21
a-naphthaleneacetic acid (NAA) (0.54 mM) with some cultures becoming highly prolific after repeated subculture. Shoots elongated in proliferating cultures, and plants were successfully acclimatized and planted into the greenhouse. The results indicate that tissue culture may be used as a means to propagate this epiphytic bromeliad species, which is being seriously affected by deforestation and habitat destruction. In addition, adventitious bud proliferation can provide a means to propagate superior genotypes.
Abstract A successful protocol for high frequency callus induction and plant regeneration from Anthurium andrea- num Linden ex Andre´ cv. Tropical half-anthers is descri- bed. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy ace- tic acid (0.1–1.0 mg/l), a-naphthalene acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylami- nopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentra- tion of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root for- mation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suit- able for callus formation while an improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzyl- aminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3 containing. 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were suc- cessfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for devel- oping half-anther culture of other Anthurium species or cultivars is discussed.