Murashige and Skoog (MS) media is a nutrient formula widely used in plant tissue culture labs to cultivate plant cells. Developed in 1962, it’s one of the most common plant growth mediums due to its effectiveness for various plant types.
Here’s a table outlining the basic MS media formula:
| Component | Concentration (mg/L) |
|---|---|
| Macronutrients | |
| Potassium Nitrate (KNO3) | 1900 |
| Ammonium Sulfate ((NH4)2SO4) | 700 |
| Calcium Chloride (CaCl2.2H2O) | 440 |
| Magnesium Sulfate (MgSO4.7H2O) | 370 |
| Potassium Phosphate Dibasic (K2HPO4) | 170 |
| Micronutrients | |
| Manganese Sulfate (MnSO4.H2O) | 20 |
| Zinc Sulfate (ZnSO4.7H2O) | 8 |
| Boric Acid (H3BO3) | 6.2 |
| Sodium Molybdate (Na2MoO4.2H2O) | 0.25 |
| Cupric Sulfate (CuSO4.5H2O) | 0.05 |
| Cobalt Chloride (CoCl2.6H2O) | 0.05 |
| Ferrous Sulfate (FeSO4.7H2O) | 27.8 |
| Vitamins | |
| Myo-Inositol | 100 |
| Thiamine Hydrochloride (B1) | 1 |
| Pyridoxine Hydrochloride (B6) | 1 |
| Nicotinic Acid | 0.5 |
| Glycine | 2 |
| Carbon Source | |
| Sucrose | 30,000 (Note: This concentration can vary depending on the application, MS media is often denoted by a number following MS indicating sucrose concentration, e.g., MS30 refers to media with 3% sucrose) |
| Other | |
| Agar (g/L) | 8 (Note: This is a solidifying agent, not essential for all applications) |
Remember, this is a basic formula, and additional components like growth regulators might be added depending on the specific needs of the plant tissue being cultured.