New basal media for half-anther culture of Anthurium andreanum

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New basal media for half-anther culture of Anthurium andreanum

Abstract A successful protocol for high frequency callus induction and plant regeneration from Anthurium andrea- num Linden ex Andre´ cv. Tropical half-anthers is descri- bed. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy ace- tic acid (0.1–1.0 mg/l), a-naphthalene acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylami- nopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentra- tion of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root for- mation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suit- able for callus formation while an improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzyl- aminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3 containing. 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were suc- cessfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for devel- oping half-anther culture of other Anthurium species or cultivars is discussed.

Micropropagation of Anthurium – MATSUMOTO 1997

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Micropropagation of Anthurium - MATSUMOTO 1997

Within the family Araceae, Anthurium is the largest, most morphologically diverse and complex genus, consisting of approximately 1000 species. Native to Central and South America, members of Anthurium are found at elevations ranging from sea level to 3000 m, most commonly in cloud forests at 1500m (Croat 1986). Plants of this herbaceaous perennial monocot are terrestrial or epiphytic. Typical of the aroids is the spadix, consisting of a multitude of unobtrusive true flowers supported by a fleshy axil. The protogynous nature of the bisexual flowers in Anthurium favors cross-pollination. The commercial flower is a combination of the spadix and a colorful modified leaf, termed spathe. Attractive foliage of some species makes anthuriums also suitable for leaf harvest and cultivation as a potted plant.

Anthurium Adventures: Propagating from Stem Cuttings

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Anthurium Adventures - Propagating from Stem Cuttings

The captivating anthurium, with its vibrant blooms and elegant foliage, is a popular indoor plant. But what if you could multiply your beloved anthurium and create more of these tropical beauties? This article explores propagating anthuriums from stem cuttings, guiding you through both water and soil propagation methods. With a little patience and these simple steps, you’ll be well on your way to an anthurium haven!

Unveiling the Magic: Propagating Anthuriums Through Tissue Culture

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Unveiling the Magic - Propagating Anthuriums Through Tissue Culture

Anthuriums, with their captivating flowers and glossy leaves, are stars of the indoor plant world. But have you ever wondered how nurseries cultivate such an abundance of these tropical beauties? The answer lies in a specialized technique called tissue culture. This article explores the world of tissue culture propagation for anthuriums, guiding you through the process and its advantages.

Propagating Pothos: From Humble Cuttings to Lush Greenery

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Propagating Pothos: From Humble Cuttings to Lush Greenery

The pothos (Epipremnum aureum) is a beloved houseplant known for its lush foliage and forgiving nature. But did you know you can easily multiply your existing pothos into a whole new generation of cascading vines? This article explores propagating pothos from stem cuttings, guiding you through both water and soil propagation methods. With a little patience and these simple steps, you’ll be a pothos-propagating pro in no time!

Propagating Corymbia citriodora ‘Scentuous’ Through Grafting

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Propagating Corymbia citriodora 'Scentuous' Through Grafting

The Corymbia citriodora ‘Scentuous,’ a captivating dwarf version of the lemon-scented gum, is prized for its manageable size and delightful fragrance. However, unlike many plants, ‘Scentuous’ isn’t propagated through stem cuttings or division. This article delves into the fascinating technique of grafting, the secret behind multiplying these lovely trees.

Gibberellic Acid‑Priming Promotes Fluoride Tolerance in a SusceptibleIndica Rice Cultivar by Regulating the Antioxidant and PhytohormoneHomeostasis

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Gibberellic Acid‑Priming Promotes Fluoride Tolerance in a SusceptibleIndica Rice Cultivar by Regulating the Antioxidant and PhytohormoneHomeostasis

Excessive utilization of groundwater for anthropogenic purposes has led to severe depletion of the water table, resulting in
contamination of fluorides from the mineral bed. Irrigation of rice seedlings with such fluoride-infested water leads to high
fluoride bioaccumulation and compromised growth physiology. In the present study, we showed that the priming of seeds
with gibberellic acid 3 (GA) alleviated prolonged fluoride-induced toxicity in the fluoride-susceptible indica rice cultivar,
IR-64 (grown in soil) by reducing the accumulation of the xenobiotic within the seedling biomass. The primed seeds showed
improved percentage of germination during fluoride stress compared to the non-primed seeds. The stressed seedlings grown
from the GA-primed seeds exhibited increased endogenous accumulation of GA and the auxin, indole-3-acetic acid which
stimulated shoot and root growth and relative water content, compared to the stressed seedlings germinated from the nonprimed
seeds. GA-priming reduced the chlorophyll degradation, affected the homeostasis of the accessory pigments and
lowered the electrolyte leakage during stress. Upon GA-priming, the fluoride-induced oxidative stress was ameliorated by
an increase in proline, anthocyanin, flavonoid and total phenolic contents, reducing power, total antioxidant capacity and
DPPH-radical scavenging activity. The altered activity of the antioxidative enzymes like catalase, ascorbate peroxidase and
guaiacol peroxidase also enabled efficient H2O2
scavenging in the stressed plants germinated from the primed seeds. Thus,
seed pre-treatment with GA promoted fluoride tolerance by activating the antioxidant machinery and elevating the endogenous
level of the two most important classes of plant growth regulators, gibberellic acid and auxin.

Linsmaier and Skoog (LS) Media

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Linsmaier and Skoog (LS) Media - Tobacco Tissue Culture

Linsmaier and Skoog (LS) Media is a versatile plant growth medium known for its effectiveness in various plant tissue culture applications, including micropropagation, organ culture, and callus culture. It provides an optimized nutrient profile, particularly beneficial for tobacco cultures.