Micropropagation

The Miniaturized Marvel of Plant Production

Micropropagation, also known as tissue culture, is the botanical equivalent of miniaturization technology. Imagine a world where a single plant can be replicated thousands of times, not through seeds or traditional cuttings, but through the magic of tiny tissues. That’s the essence of micropropagation! Scientists and growers take a sliver of a plant, a bud, or even a single cell, and introduce it to a controlled laboratory environment. Here, in a sterilized flask filled with a special nutrient broth, these miniature plant parts embark on a remarkable journey. With the help of precisely formulated growth regulators and under the watchful eye of skilled technicians, these tiny fragments multiply and differentiate, eventually transforming into complete plantlets, miniature versions of the original plant.

Micropropagation offers a treasure trove of benefits:

Rapid Replication: Produce large quantities of plants in a short period, independent of seasonal constraints.
Disease-Free Offspring: Eliminate the risk of transmitting diseases from the mother plant.
True-to-Type Traits: Ensure the new plants possess the exact characteristics of the chosen parent.
Cultivating the Rarest: Propagate endangered or commercially valuable plants that might be difficult to reproduce through traditional methods.

Micropropagation is a revolutionary technique that has transformed the world of horticulture. It’s a testament to human ingenuity and our ability to manipulate the wonders of plant biology at a microscopic scale. This technology holds immense potential for the future of agriculture, allowing us to cultivate a wider variety of plants, meet the demands of a growing population, and ensure the survival of precious plant species.

How to Propagate Abies numidica

The tiny seeds, each a promise of a majestic Algerian Fir, held their secrets tight. Months of chilling mimicry – a patient winter slumber in damp peat – preceded the anxious wait. Then, a miracle: a fragile green spear, pushing through the dark earth, a testament to perseverance. Each seedling, a tiny triumph against the odds, a vibrant emerald hope in the face of dormancy and potential failure, rewarded the gardener’s meticulous care with a breathtaking glimpse of the future: the towering, bluish-green cones of the Atlas Mountains, reborn in a humble pot.

How to Propagate Abies chensiensis Tiegh. LCLeast ConcernPopulation trend: Unknown

The Shensi fir, a pyramidal jewel of dark green, promised majestic beauty, but its propagation whispered of a gardener’s trial by fire. Each tiny seed, a stubborn fortress of dormancy, demanded a winter’s mimicry—months of chilling stratification, a gamble against rot and fungal foes. The germination, a slow, hesitant awakening, felt like coaxing life from slumber. Success, the emergence of a fragile seedling, was a hard-won victory, a testament to patience nurtured in the cold soil, a quiet triumph against the odds. The reward? A breath of the fir’s clean, resinous scent—a fragrance of perseverance, a promise of enduring beauty.

Effective callus induction and plant regeneration in callusand protoplast cultures of Nigella damascena L.

In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising:
(1) callus induction, (2) somatic embryogenesis in callus cultures with subsequent plant regeneration, and (3) isolation
and regeneration of callus-derived protoplasts. Callus development was achieved on 83–100% of hypocotyl and cotyledon
explants, whereby Murashige and Skoog medium (MS) supplemented with 3 mg L−
1 6-benzylaminopurine and 0.5 mg L−
1
α-naphthaleneacetic acid (NAA; BN medium) was more advantageous than MS with kinetin and NAA (KN medium). Histological
observations of calli revealed the presence of embryogenic zones from which somatic embryos developed on the
hormone-free medium. Plant regeneration was observed on 76–95% of calli. A high capacity to form somatic embryos and
regeneration was maintained in long-lasting cultures, i.e. even in 2 year old callus.
The obtained callus was also a good source tissue for protoplast isolation. By applying a mixture of cellulase and pectolyase,
the acceptable yield of viable protoplasts was achieved, especially from hypocotyl-derived callus maintained on BN medium.
Protoplasts embedded in an alginate matrix and cultured in modified Kao and Michayluk media re-constructed their cell
wall and re-entered mitotic divisions. About 30% of small cell aggregates formed microcalli, which, after the release from
alginate, proliferated continuously on KN and BN media, irrespective of the tissue variant used as the protoplast source.
Somatic embryo formation and plant regeneration were successful on hormone-free media. An effective plant regeneration
system of N. damascena protoplast cultures has been developed and is being reported for the first time

Unveiling the Magic: An In-Depth Exploration of Micropropagation

Micropropagation, also known as plant tissue culture, is a powerful technique for rapidly multiplying desirable plant varieties. This article delves into the intricate world of micropropagation, exploring its principles, procedures, and extensive applications. We’ll unveil the fascinating journey of transforming tiny plant tissues into a multitude of genetically identical offspring within a controlled laboratory environment.

Adventitious Bud Development and Regeneration in Tillandsia Eizii

The bromeliad Tillandsia eizii is a striking species with large, colorful, and persistent inﬂorescences that can reach 1 m in length. The value of this plant as an ornamental and its importance in cultural and religious activities has led to its over-collection in the wild. Clonal propagation via tissue culture may be a means to repopulate native stands while meeting the demands for this species as an ornamental and ceremonial plant. Adventitious bud proliferation was induced from axenically germinated seedling material. Parameters evaluated were the age of explant material at the time of transfer onto bud-induction medium, the concentration of plant growth regulators, and the period of exposure to induction medium. Light and scanning electron microscopy (SEM) established the origin and development of buds. Twelve-week-old seedling explants rapidly initiated adventitious buds after a 30-d induction period on shoot-initiation medium. Adventitious buds were induced in 40% of the explants placed on media with 2 mg l21 6-benzylaminopurine (BA) (8.88 mM) plus 0.1 mg l21
a-naphthaleneacetic acid (NAA) (0.54 mM) with some cultures becoming highly proliﬁc after repeated subculture. Shoots elongated in proliferating cultures, and plants were successfully acclimatized and planted into the greenhouse. The results indicate that tissue culture may be used as a means to propagate this epiphytic bromeliad species, which is being seriously affected by deforestation and habitat destruction. In addition, adventitious bud proliferation can provide a means to propagate superior genotypes.

New basal media for half-anther culture of Anthurium andreanum

Abstract A successful protocol for high frequency callus induction and plant regeneration from Anthurium andrea- num Linden ex Andre´ cv. Tropical half-anthers is descri- bed. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy ace- tic acid (0.1–1.0 mg/l), a-naphthalene acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylami- nopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentra- tion of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root for- mation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l a-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suit- able for callus formation while an improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l a-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzyl- aminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3 containing. 0.2 mg/l a-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were suc- cessfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for devel- oping half-anther culture of other Anthurium species or cultivars is discussed.

Micropropagation of Anthurium – MATSUMOTO 1997

Within the family Araceae, Anthurium is the largest, most morphologically diverse and complex genus, consisting of approximately 1000 species. Native to Central and South America, members of Anthurium are found at elevations ranging from sea level to 3000 m, most commonly in cloud forests at 1500m (Croat 1986). Plants of this herbaceaous perennial monocot are terrestrial or epiphytic. Typical of the aroids is the spadix, consisting of a multitude of unobtrusive true flowers supported by a fleshy axil. The protogynous nature of the bisexual flowers in Anthurium favors cross-pollination. The commercial flower is a combination of the spadix and a colorful modified leaf, termed spathe. Attractive foliage of some species makes anthuriums also suitable for leaf harvest and cultivation as a potted plant.